Journal: Vascular Pharmacology

Article Title: SARS-CoV-2 spike protein-mediated cell signaling in lung vascular cells

doi: 10.1016/j.vph.2020.106823

Figure Lengend Snippet: The SARS-CoV-2 spike protein S1 promotes the MEK phosphorylation in human cells. (A) Human pulmonary artery smooth cells ( N = 5) and (B) human pulmonary artery endothelial cells ( N = 3) were treated with recombinant full length S1 subunit of the SARS-CoV-2 spike protein (Val16 - Gln690) at 10 ng/ml for the durations indicated. Cell lysates were prepared and subjected to Western blotting using antibodies against phosphorylated MEK (p-MEK), MEK protein, phosphorylated Akt (p-Akt), and phosphorylated Stat3 (p-Stat3). Bar graphs represent means ± SEM. *Significantly different from 0 min at p < 0.05.

Article Snippet: Human pulmonary artery smooth muscle cells and human pulmonary artery endothelial cells were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA), and rat pulmonary artery smooth muscle cells were purchased from Cell Applications (San Diego, CA, USA).

Techniques: Phospho-proteomics, Recombinant, Western Blot

Journal: Vascular Pharmacology

Article Title: SARS-CoV-2 spike protein-mediated cell signaling in lung vascular cells

doi: 10.1016/j.vph.2020.106823

Figure Lengend Snippet: In rat cells, the SARS-CoV-2 spike protein S1 does not phosphorylate, but rather dephosphorylates MEK. Rat pulmonary artery smooth muscle cells were treated with recombinant full length S1 subunit of the SARS-CoV-2 spike protein (Val16 - Gln690) at 10 ng/ml for the durations indicated. Cell lysates were prepared and subjected to Western blotting using antibodies against phosphorylated MEK (p-MEK) and MEK protein. The bar graph represents means ± SEM ( N = 4). *Significantly different from 0 min at p < 0.05.

Article Snippet: Human pulmonary artery smooth muscle cells and human pulmonary artery endothelial cells were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA), and rat pulmonary artery smooth muscle cells were purchased from Cell Applications (San Diego, CA, USA).

Techniques: Recombinant, Western Blot

Journal: Vascular Pharmacology

Article Title: SARS-CoV-2 spike protein-mediated cell signaling in lung vascular cells

doi: 10.1016/j.vph.2020.106823

Figure Lengend Snippet: The SARS-CoV-2 spike protein S1 does not promote the phosphorylation of MEK in the presence of the neutralizing antibody against ACE2. Human pulmonary artery smooth muscle cells were pre-treated with the ACE2 antibody for 1 h and then treated with recombinant full length S1 subunit of the SARSCoV-2 spike protein (Val16 – Gln690) for 10 min. Cell lysates were prepared and subjected to Western blotting using antibodies against phosphorylated MEK (p-MEK) and MEK protein. The bar graph represents means ± SEM (N = 4).*Significantly different from untreated control at p < 0.05. NS denotes that the two values are not significantly different from each other at p < 0.05.

Article Snippet: Human pulmonary artery smooth muscle cells and human pulmonary artery endothelial cells were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA), and rat pulmonary artery smooth muscle cells were purchased from Cell Applications (San Diego, CA, USA).

Techniques: Phospho-proteomics, Recombinant, Western Blot, Control

Journal: Vascular Pharmacology

Article Title: SARS-CoV-2 spike protein-mediated cell signaling in lung vascular cells

doi: 10.1016/j.vph.2020.106823

Figure Lengend Snippet: The RBD only containing SARS-CoV-2 spike protein S1 does not activate the MEK phosphorylation. (A) Human pulmonary artery smooth muscle cells and (B) human pulmonary artery endothelial cells were treated with recombinant full length S1 subunit (Val16 - Gln690) or the RBD region of S1 subunit (Arg319 - Phe541) at 100 ng/ml for the durations indicated. Cell lysates were prepared and subjected to Western blotting using antibodies against phosphorylated MEK (p-MEK) and the MEK protein. Bar graphs represent means ± SEM ( N = 3). *Significantly different from 0 min control at p < 0.05.

Article Snippet: Human pulmonary artery smooth muscle cells and human pulmonary artery endothelial cells were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA), and rat pulmonary artery smooth muscle cells were purchased from Cell Applications (San Diego, CA, USA).

Techniques: Phospho-proteomics, Recombinant, Western Blot, Control